identifiler plus user guide

These improvements enhance the assay signal-to-noise ratio and simplify the interpretation of results. The y-axis is scaled to 130 RFU, and the artifacts are 111 For both kits, 1 ng of input DNA and 28 cycles of amplification were used. AmpFSTR Identifiler Direct PCR Amplification Kit USER GUIDE for use with: 200 reaction kit 1000 reaction kit Catalog Numbers 4467831 and 4408580 Publication Number 4415125 Revision K. Manufacturer: Thermo Fisher Scientific | 7 Kingsland Grange | Warrington, Cheshire WA1 4SR | United Kingdom Information in this document is subject to change without notice. The AmpFSTR() Identifiler() Plus PCR Amplification Kit is an improved version of the AmpFSTR() Identifiler() PCR Amplification Kit and amplifies the core CODIS loci: D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, and vWA. Identifiler Plus electropherogram generated from the DNA extraction, amplification and 3500xL fragment analysis of store purchased wheat germ. Transcription . The new kit includes the same primers and allelic ladder as the widely used Identifil-er Kit and harnesses next-generation PCR . By adding an additional dye, more loci can be For every chemical, read the Safety Data Sheets (SDSs) and follow the handling . Purpose The Applied Biosystems AmpFlSTR Identifiler PCR Amplification Kit User Guide The 1.0 ng target produced normalized peak height from 650 to 2100 rfu. The AmpFLSTR Identifiler PCR Amplification Kit is the single most discriminating and widely used STR based kit for human identification applications from Applied Biosystems. Table 1 AmpFlSTR Identifiler Plus PCR Amplification Kits Note: For safety and biohazard guidelines, refer to the "Safety" section in the AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide (Pub. Post-PCR purification decreases the remaining components left over from amplification, such as deoxyribonucleotide triphosphates, primers, salts, and enzymes so there is less competition during the electrokinetic injection and more DNA will go into the capillary. Quantifiler HP DNA Quantification Kit contains all the necessary reagents for the amplification, detection and quantification of two huma n-specific DNA targets. Manufacturer: Thermo Fisher Scientific | 7 Kingsland Grange | Warrington, Cheshire WA1 4SR | United Kingdom The information in this guide is subject to change without notice. Share to Tumblr. 1 manual. AmpFlSTR Identifiler Plus User Guide 7 Preface Purpose The Applied Biosystems AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide provides information about the Applied Biosystems instruments, chemistries, and software associated with the AmpFlSTR Identifiler Plus PCR Amplification Kit. 4486461) Note: For safety and biohazard guidelines, refer to the "Safety" section in the AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide (Pub. 10 AmpFlSTR Identifiler Plus User Guide Chapter 1 Overview Product overview Purpose The AmpFlSTR Identifiler Plus PCR Amplification Kit is a short tandem repeat (STR) multiplex assay that amplifies 15 tetranucleotide repeat loci and the Amelogenin gender-determining marker in a single PCR amplification: Performance Characteristics 4440211). Quality Assurance Manual, the accreditation standards under ISO/IEC 17025:2005, and the supplemental standards set by the FSL's accrediting body, as well as the . Pull-out chapters This guide is designed to allow users to pull out chapters 2, 3 . Wear appropriate protective eyewear, clothing, and gloves. Additional loci amplified in the multiplex reaction are the sex . 4486461) Note: For safety and biohazard guidelines, refer to the "Safety" section in the AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide (Pub. Optimized for casework, the AmpFLSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. Share to Twitter. Share to Pinterest. Table 1 AmpFlSTR Identifiler Plus PCR Amplification Kit (1000 Reactions) (Part no. In addition, the Identifiler Plus Master Mix includes AmpliTaq Gold DNA Polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and . The Identifiler kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards. Documents. Helps overcome PCR inhibition through the use of a high performance PCR buffer system and optimized thermal . . For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions. When post-PCR purification is used; PH's will increase, PHRs should remain . The improvements in the Identifiler Plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the Identifiler kit. no. USER GUIDE Catalog Numbers 4476135 (200 reactions), 4482815 (1,000 reactions) Publication Number 4477604 Revision E. The information in this guide is subject to change without notice. . Share via email. AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide (Pub Item Preview remove-circle Share or Embed This Item. Revision Date Description A May 2001 New document. Product Overview. 4440211). The two peaks at 176.82 bp in D8S1179 and 156.05 bp in vWA are consistent with the ID_D8-1 and ID_vWA-2 artifacts described in this document. CLA Identifiler Plus and Identifiler Direct PCR Amplification Kits USER GUIDE for Cell Line Authentication for use with: SeqStudio Genetic Analyzer 3500/3500xL Genetic Analyzer 3730/3730xl DNA Analyzer (48-capillary) 3130/3130xl Genetic Analyzer for use with: Microsatellite Analysis Software GeneMapper Software Catalog Numbers . no. Note: For safety and biohazard guidelines, refer to the "Safety" section in the Identifiler Plus PCR Amplification Kit User Guide (Part no. Share to Facebook. AmpFlSTR Identifiler Plus PCR Amplification Kit User`s Guide DISC Catalog number: 4322288. Unless stated otherwise, the protocols in the Identifiler Plus Kit User Guide were followed (15). Price (USD) 4440211. The combination of the 15 loci are consistent with several worldwide database recommendations. AmpFlSTR Identifiler PCR Amplification Kitfor use with: 200 reaction kit (Part no. This is the User Guide for the AmpFLSTR Identifiler Plus PCR Amplification Kit. ERR#1200 - This item cannot be ordered online. USER GUIDE For Research, Forensic, or Paternity Use Only. 4440211). Before using this product, read and understand the information the "Safety" appendix in this document. FBS21 - Identifiler Plus Interpretation Guidelines Page 1 of 34 Document Control Number: 2519 Approved By: Jennifer Zeffer Revision: 2 Issue Date: 2/12/2015 3:44:14 PM . 4322288) Publication Number 4323291 Rev. The Identifiler kit is unable to amplify a blood sample from a leather glove. Identifiler Plus kits. Not for diagnostic procedures. Save to list. Five-Dye DNA Fragment Analysis The Identifiler kit uses a five-dye fluorescent system for automated DNA fragment analysis. For every chemical, read the Safety Data Sheets (SDSs) and follow the handling . (STR kits are For Forensic or Paternity Use Only.) Share to Reddit. B May 2001 Update GeneAmp System 9700 part numbers. The PCR amplification reac-tion mixture was prepared in a volume of 25 lL containing 10 lLofAmpF'STR Identifiler Plus Master Mix, 5 lLof AmpF'STR Identifiler Plus Primer Set, and a maximum vol-ume of 10 lL of target DNA. loci are consistent with the AmpFlSTR SGM Plus PCR Amplification Kit. The recommended target DNA input for amplification using the Identifiler Plus kit at 28 cycles is approximately 1.0 ng. Table 1 AmpFlSTR Identifiler Plus PCR Amplification Kit (1000 Reactions) (Part no. AmpFlSTR Identifiler PCR Amplification Kit User Guide 9 About This Guide IMPORTANT! 10 AmpFlSTR Identifiler Plus User Guide Chapter 1 Overview Product overview Purpose The AmpFlSTR Identifiler Plus PCR Amplification Kit is a short tandem repeat (STR) multiplex assay that amplifies 15 tetranucleotide repeat loci and the Amelogenin gender-determining marker in a single PCR amplification: Here, we review the key performance measures of three new STR amplification systems with 6-dye chemistry, namely, the Investigator 24plex QS Kit from QIAGEN, the GlobalFiler PCR Amplification Kit from Applied Biosystems, and the PowerPlex Fusion 6C System from Promega. 4387746 Publication Number 4391294 Revision E Our results have demonstrated that GlobalFiler displays the highest . . For Forensic or Paternity Use Only. and the Identifiler Plus kit including SNP-specific primers for the vWA, D16S539, AMEL, D2S441, D22S1045, and D8S1179 loci. 4440211). For Forensic or Paternity Use Only. Quantifiler Duo DNA Quantification Kit For use with: Catalog no. example, the Identifiler Plus, NGM SElect or the GlobalFiler PCR Amplification Kits. The primers used in the kits are: For all loci except AMELThe same primer sequences as the NGM SElect kit and the Identifiler Plus kit including SNP-specific primers for the vWA, Wear With its enhanced formulation, the Identifiler Plus kit helps deliver a full profile. JRevision Date August 2012. The Applied Biosystems AmpFlSTR Identifiler PCR Amplification Kit User Guide provides information about the Applied Biosystems instruments, chemistries, and software associated with the AmpF lSTR Identifiler PCR Amplification Kit. USER GUIDE. no. For every chemical, read the Safety Data Sheets (SDSs) and follow the handling instructions. The AmpFSTR() Identifiler() Plus PCR Amplification Kit is an improved version of the AmpFSTR() Identifiler() PCR Amplification Kit and amplifies the core CODIS loci: D3S1358, D5S818 . study (sensitivity_3) was performed to evaluate the Identifiler Plus kit under the current laboratory conditions.

Florence Dining Chair, 240v To 12v Transformer Heavy Duty, Bratislava Real Estate, Carhartt Wip Regular Cargo Pant Camo, Service Fabric Vs Kubernetes, Formal Trousers For Girls, Iso Commercial Auto Class Codes, Whirlpool Side By Side Ice Maker Not Working,